I finally went to the Arabidopsis database and looked up the HOTHEAD gene mentioned in my previous post. Here is the database’s information on the gene. The detailed annotation of the gene, describes what is known or predicted about it:

  • involved in cell-cell signaling (inferred from mutant phenotype)
  • located in extracellular region (inferred from sequence or structural similarity)
  • has mandelonitrile lyase activity (inferred from sequence or structural similarity)
  • functions in FAD binding (inferred from sequence or structural similarity)

The predicted mandelonitrile lyase activity suggests that the gene may catylse the reaction of mandelonitrile = cyanide + benzaldehyde. Here are some details of the enzyme from Black Cherry.

FUNCTION: Involved in cyanogenis, the release of HCN from injured tissues. Catalyzes the stereospecific addition of HCN to a variety of aldehydes in vitro. It is a major seed constituent, and could have the additional role of a storage form for reduced nitrogen. CATALYTIC ACTIVITY: Mandelonitrile = cyanide + benzaldehyde. COFACTOR: FAD

None of this seems to help me get a clear picture right now. Anybody got any idea?


I’m not sure I understand the state of the art, but: they have the sequence, right? Can they not engineer some E. coli to include the gene and manufacture its product in vitro? And then, like, mix it with mandelonitrile to see if it catalyzes the predicted reaction?

We have the same Pruitt’s results in respect genetic reversion on Arabidopsis It was published: П.П.Даряев, Е.А.Леонова, 2003, Странный мир волновой генетики. Журнал «Сознание и физическая реальность», Ñ‚.8, â„–6, с.27-40.

The same how? Sorry, but I don’t know Russian.

You can use babelfish ( to translate text or web pages from one language to another, inlcuing from Russian to English

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This page contains a single entry by Reed A. Cartwright published on March 25, 2005 12:25 AM.

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